MICROSATELLITE POLYMORPHISM OF THE NATURAL AND DOMESTICATED AZOV AND BLACK SEA POPULATIONS OF STELLATE STURGEON ACIPENSER STELLATUS
Abstract
On the basis of analysis of five microsatellite loci (An20, AoxD161, AoxD165, Afug41, Afug51), the special characteristics of intraspecies polymorphism of the natural and domesticated populations of the Azov and Black Sea stellate sturgeonare identified.Significant divergence between the individuals in different groups of broodstocks and between the individuals in broodstocks and in natural populations, according to the established distribution of frequenciesof STR-alleles (p < 0.001), is presented. By contrast, the groups of fish individuals from the Black Sea and the Sea of Azov are only slightly differentiated (Fst = 0.007). It is evident that intraspecies population composition of the Azov and Black Sea stellate sturgeon underwent extensive changes, resulting from anthropogenic pressure.
About the Authors
N. N. Timoshkina
Azov Fisheries Research Institute
Russian Federation
Russian Federation
Rostov-on-Don, 344002
N. A. Nebesikhina
Azov Fisheries Research Institute
Russian Federation
Russian Federation
Rostov-on-Don, 344002
E. A. Ivanova
Azov Fisheries Research Institute
Russian Federation
Russian Federation
Rostov-on-Don, 344002
A. G. Lepeshkov
Azov Fisheries Research Institute
Russian Federation
Russian Federation
Rostov-on-Don, 344002
A. E. Barmintseva
Russian Federal Research Institute of Fisheries and Oceanography
Russian Federation
Russian Federation
Moscow, 107140
References
1. Берг Л.С. Рыбы пресных вод СССР и сопредельных стран. Ч.1. Л.; М.: Изд-во АН СССР, 1948. 514 с.
2. Макаров Э.В. Проблемы сохранения и восстановления популяций осетровых и перспективы развития осетроводства в Азовском бассейне: Автореф. дис. … докт. биол. наук. М.: АзНИИРХ, 2000. 70 с.
3. Мюге Н.С. , Барминцева А.Е., Расторгуев С.М. и др. Полиморфизм контрольного региона митохондриальной ДНК восьми видов осетровых и разработка системы ДНК-идентификации видов // Генетика. 2008. Т. 44. №7. С. 1–7.
4. Aljanabi Salah M., Martinez I. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques // Nucl. Acids Res. 1999. V. 25. № 22. P. 4692–4693.
5. Bacalbasa-Dobrovici N. Endangered migratory sturgeons of the lower Danube River and its delta // Environ. Biol. Fish. 1997. V. 48. P. 201–207.
6. Cornuet J.M., Luikart G. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data // Genetics. 1996. V. 144. №4. P. 2001–2014.
7. Earl D.A., Von Holdt B.M. Structure Harvester: a website and program for visualizing STRUCTURE output and implementing the Evanno method // Cons. Genet. Res. 2012. V. 4. P. 359–361.
8. Henderson-Arzahola A., King T.L. Novel microsatellite markers fo Atlantic sturgeon (Acipenser oxyrinchus) population delineation and broodstock management // Mol. Ecol. Notes. 2002. V. 2. P. 437–439.
9. Hensel K., Holcik J. Past and current status of sturgeons in the upper and middle Danube – Sturgeon Biodiversity and Conservation. Dordrecht: Kluwer Acad. Publ., 1997. Р. 185–200.
10. Nei M. Bottlenecks, genetic polymorphism and speciation // Genetics. 2005. V. 170. № 1. P. 1–4.
11. Peakall R., Smouse P.E. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update // Bioinformatics. 2012. V. 28. № 19. P. 2537–2539.
12. Pritchard J.K., Stephens M., Donnelly P. Inference of population structure using multilocus genotype data // Genetics. 2000. V. 155. № 2. P. 945–959.
13. Raymakers С. CITES, the Convention on International Trade in Endangered Species of Wild Fauna and Flora: its role in the conservation of Acipenseriformes // J. Appl. Ichthyol. 2006. V. 22. Suppl. 1. P. 53–63.
14. Rousset F. Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux // Mol. Ecol. 2008. № 8. P. 103–110.
15. Welsh A., May B. Development and standartization of disomic microsatellite markers for lake sturgeon genetic studies // J. Appl. Ichthyol. 2006. V. 22. P. 337– 344.
16. Zane L., Patarnello T., Ludwig А. et al. Isolation and characterization of microsatellites in the Adriatic sturgeon (Acipenser naccarii) // Mol. Ecol. Notes. 2002. V. 2. P. 586– 588.
Review
For citations:
Timoshkina N.N., Nebesikhina N.A., Ivanova E.A., Lepeshkov A.G., Barmintseva A.E. MICROSATELLITE POLYMORPHISM OF THE NATURAL AND DOMESTICATED AZOV AND BLACK SEA POPULATIONS OF STELLATE STURGEON ACIPENSER STELLATUS. Problems of Fisheries. 2018;19(4):491-499. (In Russ.)
Email this article 